Principle and Operation of Shimadzu ISR 2600 Plus Integrating Sphere Accessory

Summary

Join Jian, an application chemist at Shimadzu Center for Application Solutions, as he walks us through the use of the ISR 2600 Plus integrating sphere. This innovative device ensures complete detection of transmitted light, overcoming issues caused by refractive index differences between air and sample. By accommodating total and diffuse transmissions, along with total and diffuse reflections, the ISR 2600 Plus optimizes optical measurements. Learn about its unique operation, optical systems, and the four critical measurement modes to unlock its full potential.

Highlights

• Integrating spheres overcome detection issues caused by refractive index differences ⚙️.
• Total transmitted light includes both direct and diffused light paths 📡.
• The ISR 2600 Plus features dual beams entering at different angles for thorough detection 🔭.
• Four measurement modes cater to different light interaction scenarios with samples ☀️.
• Routine baseline correction is crucial for accurate measurements 🧪.

Key Takeaways

• Understand the role of integrating spheres in capturing all transmitted light 🌟.
• Learn about the four modes: total transmission, diffuse transmission, diffuse reflection, and total reflection 🔍.
• Discover how refractive indexes alter light's path in standard setups 🌈.
• Find out how the ISR 2600 Plus manages light refraction and scattering 🎯.
• Optimizing optical measurements is made easier with proper setup 🔧.

Overview

The video unravels the challenges involved in measuring transmitted light when dealing with different refractive indexes. The introduction of the integrating sphere addresses the problem by capturing all transmitted and reflected light no matter the direction it travels. This ensures no data loss and enhances measurement accuracy.

Jian explains the workings of Shimadzu's ISR 2600 Plus with insightful detail, focusing on its twin light beam system. The device is showcased to manage light interaction effectively, considering refraction and reflection phenomena. This versatile equipment caters to comprehensive optical analysis, assisting in various scientific endeavors.

Step-by-step instructions on operating the ISR 2600 Plus are provided, detailing the four measurement modes it offers: total transmission, diffuse transmission, diffuse reflection, and total reflection. The process of conducting baseline corrections and adjusting the equipment for accurate readings is emphasized, showcasing the device's user-friendly design.

Chapters

• 00:00 - 00:30: Introduction and Purpose of an Integrating Sphere The chapter introduces the concept and purpose of an integrating sphere. It begins with Jian, an application chemist from Gemato Center for Application Solutions, introducing the ISR 3600 plus integrating sphere. The primary purpose of an integrating sphere in the standard direct transmission configuration is to focus light on a light-sensitive element.
• 00:30 - 01:30: Types of Light Interaction with a Sample When no sample is present, light hits the detector directly, but placing a sample shifts the focal point due to different refractive indexes, causing some light to be undetected. An integrating sphere can capture all transmitted light. Light interactions with a sample include direct transmission, where light passes through the sample in the same direction it originally traveled.
• 01:30 - 02:00: Understanding the Optical System of ISR-2600 Plus The chapter 'Understanding the Optical System of ISR-2600 Plus' explains the behavior of light as it interacts with surfaces. Key concepts include refraction, scattering, specular reflection, and diffuse reflection. Refraction and scattering result in transmitted light, and the combination of this transmitted light is referred to as the total transmitted light. On the other hand, reflection can occur as specular reflection, where the angle of reflection equals the angle of incidence, or as diffuse reflection. Together, these make up the total reflected light.
• 02:00 - 04:00: Measurement Modes Demonstration This chapter introduces the ISR-2600 plus integrating sphere and its Optical system. It explains that the system contains two light beams: the sample light beam and the reference light beam. The sample light beam enters the sphere perpendicular to the sample reflectance window, while the reference light beam enters at an incident angle of 8 degrees. Both beams are detected by a central detector within the sphere. Additionally, the video will demonstrate four different measurement modes.

Principle and Operation of Shimadzu ISR 2600 Plus Integrating Sphere Accessory Transcription

• 00:00 - 00:30 [Music] thank you hello I'm Jian an application chemist of gemato Center for application Solutions in this video I will demonstrate how to use the ISR 3600 plus integrating sphere firstly what is the purpose of an integrating sphere in the standard direct transmission configuration the light focuses on the light sensitive
• 00:30 - 01:00 surface of the detector however when a sample is placed the difference in the refractive indexes of the air and Sample causes the focal point to shift away thus some of the transmitted light is undetected with an integrating sphere all of the transmitted light can be captured and detected there are several ways light interacts with a sample it can pass through the sample at the same direction it travels towards it causing direct transmitted light it can also pass through in a
• 01:00 - 01:30 different direction due to refraction and scattering causing the few strands mitted light the sum of both types of transmitted light is known as total transmitted light light can also reflect from the surface of a sample if the angle of reflection is equal to the angle of incidence it causes specular reflected light otherwise it causes diffuse reflected light the sum of both types of reflected light is known as total reflected light
• 01:30 - 02:00 to understand how to use the isr-2600 plus integrating sphere we have to first understand its Optical system there are two light beams the sample light beam which enters the sphere perpendicular to the sample reflectance window and the reference Light Beam which enters at an incident angle of 8 degrees both light beams are detected by the detector at the center of the sphere this video will demonstrate the following four measurement modes with
• 02:00 - 02:30 the isr-2600 plus integrating sphere total transmission diffuse transmission diffuse reflection and finally total reflection let's start with total transmission before any measurement place the standard white plates at both the sample and reference reflectance measurement windows then run a baseline correction this applies for all measurement modes for total transmission measurement place
• 02:30 - 03:00 the sample at the sample light entrance window using the appropriate sample holder then start the measurement next let's look at diffuse transmission measurement after Baseline correction place the sample at the sample light entrance window unlike the previous measurement remove the standard white plate at the sample reflectance window to eliminate direct transmitted light then start the measurement the third measurement mode is for diffuse reflection
• 03:00 - 03:30 after Baseline correction replace the standard white plate at the sample reflectance window with your sample any specular reflected light will be removed from the sample light entrance window leaving behind a fuse reflected light in the sphere finally we have the total reflection measurement after Baseline correction replace the standard white plate at the reference reflectance window with your sample the incident angle of 8 degrees causes the
• 03:30 - 04:00 specular reflected light to bounce around the inner walls of the sphere ensuring that it is detected thank you for watching this video [Music] excellence in science shimazu